Digital Holography Microscope

HO-DHM-UT01

 

Description

HO-DHM-UT01 is a transmission type upright digital holography microscopes with balanced afocal configuration that uses infinity corrected plan achromatic objectives in both reference and object beams. A common tube lens is provided for achieving afocal imaging configuration with minimal aberrations. Sony make CMOS sensor is used for the recording of holographic images. A linearly polarized 650nm diode laser is used for illumination in the DHM system. A high bright LED illuminated brightfield observation with binocular viewing is also integrated in the system. Since users may not be familiar with phase images, the LED illumination can be used first to focus the cell sample as desired, followed by recording of the phase image using laser illumination.

In addition to the object beam objective, two low power objectives are also provided for larger FOV in brightfield observations. The nosepiece has a triple turret mechanism for holding three microscope objectives. The user can easily switch the objectives manually. As the low power objectives have large FOV, the user can use them first to locate the desired area of sample. A manually operated XY sample stage is provided for scanning the sample area. Once the sample area is located, the user can change the low power objective to high power objective for the magnified image of selected area.

A manually operated shutter with source selector switch is provided near the eyepiece for the safety of eyes by blocking the laser light to the eyepiece.

Once the desired area for phase imaging is selected in brighfield mode, the user can close the manual shutter and the system will be ready for QPI.

A complete user friendly software (Digital HM_V01) with laptop is provided with system for the recording and reconstruction of the images. The software uses patented unique single shot high resolution method for the phase image reconstruction.

Digital holographic microscope with user specified high end microscope objectives (Plan apochromatic, Plan fluorite, Extra-long WD etc.) may also be made available upon special requests.

Features

    Unique single shot high resolution and accurate quantitative imaging capability (patented technology).

    No phase shifting required thus removing the need for piezo stages.

    Quantitative phase imaging without staining of cells.

    Full diffraction limited resolution performance.

    Digital holography as well as transmitted LED bright field observation.

Applications

    Quantitative study of cellular dynamics.

    Imaging of various cell types, including SKOV-3 ovarian cancer cells, fibroblast cells, testate amoeba, diatom skeletons, and red blood cells.

    3D imaging and statistics of RBCs in multiple deformation states.

    Evolution of Physiological parameters of different cells.

    Non-destructive analysis of Living cells without any contrast agent: Cell number, confluence, proliferation, cell death, migration and viability.

    Visualization of drug induced morphology changes.

    Visualization of stained or unstained / unprocessed cells.

    Toxicity studies

    Diagnosis of diabetics and to evaluate long-term glycemic controls in patients with diabetes.

    Resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders.

    Screening and diagnostics material science.

Digital holographic microscopy is an emerging modality that offers capability of quantitative phase imaging (QPI) of transparent unstained cells in their most natural state. While phasesensitive imaging methodologies such as dark-field, phase contrast and differential interference contrast are known for several decades, they cannot provide quantitative phase information. DHM can achieve this by use of interferometric imaging concept. A schematic DHM system is shown in Fig. 1:

Fig.1: Schematic of a balanced DHM system that works on the interferometric imaging principle. The phase image is obtained by digital processing of the interference signal recorded using an array sensor.

SF : Spatial filter, BS : Beamsplitter, O : Object beam, R : Reference beam, MO1 / MO2 :Microscope Objectives